BIOGRAPHICAL SKETCH Provide the following information for the key personnel in the order listed for Form Page 2. Follow the sample format on for each person. (See attached sample). DO NOT EXCEED FOUR PAGES.
NAME Jansson, Ingela		POSITION TITLE Assistant Professor of Pharmacology
EDUCATION/TRAINING  (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION	DEGREE (if applicable)		YEAR(s)	FIELD OF STUDY
University of Stockholm	B.A.		1963	Pharmacy
University of Stockholm	B.A.		1969	Biochemistry
University of Stockholm	Ph.D.		1977	Biochemistry

 

  RESEARCH AND PROFESSIONAL EXPERIENCE:  NOTE: The Biographical Sketch may not exceed four pages. Items A and B (together) may not exceed two of the four-page limit.  Follow the formats and instructions on the attached sample.

 

A. Professional Experience

1963-1966      Research Assistant, The Research Institute of National Swedish Defense

1967-1972      Research Associate, Department of Biochemistry, Univ. of Stockholm., Sweden.

1972-1973      Research Assoc.Dept. of Biochemistry, Univ. of Texas, SW Medical School, Dallas, TX.

1973-1978      Res. Assoc. Dept. of Pharmacology, Yale University School of Medicine, New Haven, CT

1978-Present     Asst. Professor, Dept. of Pharmacology, Univ. of Connecticut Health Ctr, CT

 

Awards and Other Professional Activities:

1983-Present  Member, American Association of Biochemistry and Molecular Biology.

 

B. Publications (From list of more than 60 full length papers)

D. Choudhary, I. Jansson, K. Rezaul, D.K.M. Han M., Sarfarazi and J.B. Schenkman. Cyp1b1 protein in the mouse eye during development: An immunohistochemical study. Drug Metab. Dispos. In Press (2007).

E. G. Hvastkovs, M. So, S. Krishnan, B. Bajrami, M. Tarun, I. Jansson, J. B. Schenkman, and James F. Rusling "Electrochemiluminescent           arrays for cytochrome P450-activated genotoxicity screening. DNA damage from benzo[a]pyrene metabolites", Anal. Chem:                         79,1897-1906 (2007).

D. Choudhary, I. Jansson, M. Sarfarazi and J.B. Schenkman. Physiological Significance and Expression of P450s in the developing eye. Drug Metabolism Reviews 38:337-352 (2006).

D. Choudhary, I. Jansson, I. Stoilov, M. Sarfarazi, and J.B. Schenkman. Expression patterns of mouse and human CYP orthologs (families 1-4) during development and in different adult tissues. Arch. Biochem. Biophys. 436:50-61 (2005).

B. Wang, I. Jansson, J. B. Schenkman and J. F. Rusling. Detecting enzymes that generate genotoxic benzo[a]pyrene metabolites using sensor          arrays. Anal. Chem. 37:1361-1367. (2005).

I. Stoilov, T. Razie, I. Jansson, J. B. Schenkman and M. Sarfarazi. Expression of cytochrome P4501b1 (Cyp1b1) during early mouse development. Mol. Vision 10: 629-636 (2004).

D. Choudhary, I. Jansson, I. Stoilov, M. Sarfarazi and J. B. Schenkman. Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1B1. Drug Metab. Dispos. 32: 840-867 (2004).

D. Choudhary., I. Jansson, J.B. Schenkman, M Sarfarazi., and I Stoilov. Comparative expression profiling of 40 mouse cytochrome p450 genes in embryonic and adult tissues. Arch Biochem. Biophys. 414: 91-100 (2003).

C. Estavillo, Z. Lu, I. Jansson, J.B. Schenkman and J.F. Rusling. Epoxidation of styrene by human cyt P450 1A2 by thin film electrolyis and peroxide activation compared to solution reactions. Biophys. Chem. 104:291-296 (2003) 

J.B. Schenkman, I. Jansson, Y. Lvov, J.F. Rusling, S. Boussaad, and N.J. Tao. Charge-dependent sidedness of cytochrome P450 forms studied by quartz crystal microbalance and atomic force microscopy. Arch. Biochem. Biophys. 385:78-87 (2001).

I. Stoilov, I. Jansson, M. Sarfarazi, and J.B. Schenkman. Roles of cytochrome P450 in development. Drug Metab. Drug Interac. 18: 1-23 (2001).

I. Jansson, I. Stoilov, M. Sarfarazi, and J.B. Schenkman. Enhanced expression of CYP1B1 in E. coli. Toxicology, 144:211-219 (2000).

Z. Lu, Yuri Lvov, Ingela Jansson, John B. Schenkman, and James F. Rusling,  "Electroactive Films of Alternately Layered Polycations and Iron-sulfur Protein Putidaredoxin on Gold", J. Coll. Interface Science 224:162-168 (2000).)

J.B. Schenkman and I. Jansson. Interactions between cytochrome P450 and cytochrome b₅. Drug Metab. Rev. 31:351-364 (1999).

 I. Jansson and J.B. Schenkman (1996). Substrate influence on the interaction between cytochrome P450 and cytochrome b₅ in microsomes. Arch. Biochem. Biophys. 325:265-269.

 I. Jansson, J.E. Mole and J.B. Schenkman (1995). The isolation and comparison of multiple forms of CYP2B from untreated and phenobarbital-treated rabbit liver microsomes. Arch. Biochem. Biophys. 316:275-284.

 J.B. Schenkman, A.I. Voznesensky and I. Jansson (1994). Influence of ionic strength on the P450 monooxygenase reaction and role of cytochrome b₅ in the process. Arch. Biochem. Biophys. 314:234-241.

I. Jansson, M. Curti, P.M. Epstein, J.A. Peterson and J.B. Schenkman (1990). Relationship between phosphorylation and cytochrome P450 destruction. Arch. Biochem. Biophys. 282:285-292.

D. Kupfer, I. Jansson, L.V. Favreau, A.D. Theoharides, and J.B. Schenkman (1988) Regioselective hydroxylation of prostaglandins by constitutive forms of cytochrome P450 from rat liver. Formation of a novel metabolite by a female specific P450. Arch. Biochem. Biophys. 259:441-448.

 P.M. Epstein, M. Curti, I. Jansson, C-K Huang and J.B. Schenkman (1989). Phosphorylation of cytochrome P450: Regulation by cytochrome b₅. Arch. Biochem. Biophys. 271: 424-432.

 I. Jansson, P.M. Epstein, S. Bains and J.B. Schenkman (1987). Inverse relationship between cytochrome P450 phosphorylation and complexation with cytochrome b₅. Arch. Biochem. Biophys. 259: 441-448.

 I. Jansson and J.B. Schenkman (1987). Influence of cytochrome b₅ on the Stoichiometry of the different oxidative reactions catalyzed by liver microsomal cytochrome P450. Drug Metab. Dispos. 15:344-348.

J.B. Schenkman, L.V. Favreau, J. Mole, D.L. Kreutzer and I. Jansson (1987) Fingerprinting rat liver microsomal cytochromes P450 as a means of delineating sexually distinctive forms. Arch. Toxicol.:60:43-51.

P.P. Tamburini, I. Jansson, L.V. Favreau, W.L. Backes, and J.B. Schenkman (1986) Differences in the spectral interactions between NADPH-cytochrome P450 reductase and a series of cytochrome P450 enzymes. Biochem. Biophys. Res. Commun. 137:437-442.

W.L.Backes, P.P.Tamburini, I. Jansson, G.G. Gibson, S.G. Sligar, and J.B. Schenkman (1985) Kinetics of cytochrome P450 reduction: Evidence for faster reduction of the high spin ferric state. Biochemistry 24:5130-5136.

W.L. Backes, I. Jansson, J.E. Mole, G.G. Gibson, and J.B. Schenkman (1985) Isolation and comparison of four cytochrome P450 enzymes from phenobarbital-induced rat liver. Three forms possessing identical NH₂-terminal sequences. Pharmacology 31:155-169.

I. Jansson, P.P. Tamburini, L.V. Favreau, and J.B. Schenkman (1985) The interaction of cytochrome b₅ with four cytochrome P450 enzymes from the untreated rat. Drug Metab. Dispos. 13:453-458.

I. Jansson, J. Mole and J.B. Schenkman (1985) Purification and characterization of a new form (RLM2) of rat liver cytochrome P450 from untreated rat. J. Biol. Chem. 260:7084-7093.

F.W. Sunderman, Jr., B.L.K. Mangold, S.H.Y. Wong, S.K. Shen, M.C. Reid, and I. Jansson (1983) High performance size-exclusion chromatography of ⁶³NiCl₂ -treated rats. Res. Comm. Pathol. Pharmacol. 39:477-492.

J.B. Schenkman, I. Jansson, W.L.Backes, K.C. Chen, and C. Smith (1982) Dissection of cytochrome P450 isozymes (RLM) from fractions of untreated rat iver microsomal proteins. Biochem. Biophys. Res. Commun. 107:1517-1523.

I. Jansson, and J.B. Schenkman (1981) Stoichiometry of aminopyrine demethylation with and without NADH synergism. Drug Metab. Dispos. 9:461-465.

S.R. Keyes, J.A. Alfano, I. Jansson, and D.L. Cinto (1979) Rat liver microsomal elongation of fatty acids. Possible involvement of cytochrome b₅. J. Biol. Chem. 254:7778-7784.

G. Powis and I. Jansson (1979) Stoichiometry of mixed function oxidase. Pharmacol. Ther. 7:297-311.

J.B. Schenkman I. Jansson, G. Powis, and H. Kappus (1979) Active oxygen in liver microsomes. Mechanism of epinephrine oxidation. Mol. Pharmacol. 15:428-438.

 

C. Ongoing Research Support

            "Interaction between cytochrome b₅ and NADPH-cytochrome P450 reductase with cytochrome P450 2B4 and role of cytochrome b₅ in the monooxygenase reaction." The goal of this study is to examine the topology of the cytochrome P450 and identify sites for the docking and electron transfer between redox partners in the monooxygenase reactions. We have been using quartz crystal microbalance  (QCM) and atomic force microscopy (AFM) in this study and have been attempting to also develop functional biosensors and biocatalysts utilizing cytochrome P450 monooxygenase forms. This study has been supported by funds from grant RO1 ES03154 (2004-2008), PI James F. Rusling, on which Dr. Jansson is an investigator.

 

          “Metabolic Analysis of potential glaucomatous substrates by wild-type and mutant forms of cytochrome P450 1B1.” Supported by grants from The Glaucoma Foundation (1999, 2000), P.I., M. Sarfarazi., J.B. Schenkman Co-P.I. These grants provided the back-drop for an NIH proposal  funded by NIH, R01 EY11095 (2006-2011), “Molecular genetics of primary congenital glaucoma.”, P.I. Mansoor Sarfarazi, Co-P.I. John B.

Schenkman, Ingela Jansson, Investigator. With support from this grant we have been able to prepare the CYP1B1 cDNA , amplify it and and prepare wild-type and mutant cDNA with 5’- and 3’- restriction enzyme sites needed for insertion into a pCWori expression vector. CYP1B1 and mutant forms G61E and R469W have been produced for studying the effects of mutation on biochemical properties. Those two point mutations appear in families with the PCG phenotype and show incomplete penetrance.